We evaluated cytogenetic results occurring with first trimester pregnancy loss, and assessed the type and frequency of chromosomal abnormalities after assisted reproductive treatment (ART) and compared them with a control group. We also compared the rate of chromosomal abnormalities according to infertility causes in ICSI group.
Methods: A retrospective cohort analysis was made of all patients who were referred to the Genetics Laboratory of Fertility Center of CHA Gangnam Medical Center from 2005 to 2009 because of clinical abortion with a subsequent dilation and evacuation (D&E) performed, and patients were grouped by type of conception as follows: conventional IVF (in vitro fertilization) (n=114), ICSI (intracytoplasmic sperm injection) (n=140), and control (natural conception or intrauterine insemination [IUI]) (n=128).
Statistical analysis was performed using SPSS software.
Results: A total 406 specimens were referred to laboratory, ten abortuses were excluded, and in 14 cases, we did not get any spontaneous metaphase, chromosomal constitutions of 382 specimens were successfully obtained with conventional cytogenetic methods. Overall, 52.62% of the miscarriages were found to be cytogenetically abnormal among all patients, the frequency was 48.4% in the control group, 54.3% of miscarriages after ICSI and 55.3% after conventional IVF (p=0.503).
The most prevalent abnormalities were autosomal trisomy, however, nine (11.69%) sex chromosome aneuploidy were noted in the ICSI group vs. four (6.45%) and two (3.23%) cases in the conventional IVF group and control group.
We compared chromosomal abnormalities of miscarriages after ICSI according to infertility factor. 55.71% underwent ICSI due to male factors, 44.29% due to non-male factors.
ICSI group having male factors showed significantly higher risk of chromosomal abnormalities than ICSI group having non-male factors (65.8% vs. 34.2%, p=0.009, odds ratio=1.529, 95% CI=1.092-2.141).
Conclusions: No increased risk of chromosomal abnormalities due to ART was found with the exception of a greater number of sex chromosomal abnormalities in the ICSI group with male factor infertility.
Therefore, these alterations could be correlated with the underlying parental risk of abnormalities and not with the ICSI procedure itself.
Author: Ji won KimWoo Sik LeeTae Ki YoonHyun Ha SeokJung Hyun ChoYou Shin KimSang Woo LyuSung Han Shim
Credits/Source: BMC Medical Genetics 2010, 11:153
Showing posts with label icsi. Show all posts
Showing posts with label icsi. Show all posts
Wednesday, November 3
Monday, December 14
ICSI
At the Berkley Center for Reproductive Wellness we have been treating male factor infertility for many years based on the knowledge of 3000 years of traditional Chinese medicine.
Acupuncture and herbal medicine in conjunction with certain vitamins and amino acids promote significant change in pathologic sperm. We have had excellent results in improving sperm count, improving morphology and motility as well as reducing sperm DNA fragmentation percentages.
The importance of this cannot be overstated. In the typical IVF Center, doctors will note that sperm quality is important in that in its pathological state in may not be able to penetrate the egg and facilitate fertilization. This can be overcome with intra-cytoplasmic-sperm-injection or ICSI. While fertilization with ICSI is possible, a good embryo quite often is not produced and pregnancy does not ensue.
A ‘good’ embryo is created by combining a ‘good’ egg with ‘good’ sperm. Embryos created with poor quality sperm even if fertilization occurs via ICSI are, in my opinion, based on thirteen years of clinical experience, one of the main reasons for failed IVF cycles including donor egg transfers even though fertilization with ICSI is facilitated.
Frequently the doctor will report that the embryo is “beautiful”, yet the cycle fails. You can no more judge the quality of an embryo by visualization than one can judge the health of a person by visualization. For example, the healthiest looking man can be HIV positive. Or, the healthiest looking woman can have breast cancer. One cannot judge a book by its cover.
The best and most reliable means of improving fertility outcomes either as a result of intercourse, IUI, or IVF is to first improve the quality of the components that are responsible for the creation of the embryo; sperm and egg.
See studies below for more information.
Fertility and Sterility_ Vol. 92, No. 4, October 2009 0015-0282/09/$36.00
Copyright ยช2009 American Society for Reproductive Medicine, Published by Elsevier Inc. doi:10.1016/j.fertnstert.2009.02.041
Jian Pei, Ph.D.,a,b Erwin Strehler, M.D.,b Ulrich Noss, M.D.,c Markus Abt, Ph.D.,d
Paola Piomboni, Ph.D.,e Baccio Baccetti, Ph.D.,e and Karl Sterzik, M.D.b
a Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; b Christian-
Lauritzen-Institut, Ulm, Germany; c In Vitro Fertilization Center Munich, Munich, Germany; d Institute for Mathematics,
University of Augsburg, Augsburg, Germany; and e Department of Paediatrics, Obstetrics and Reproductive Medicine, Section
of Biology, Siena University, Siena, Italy
Acupuncture and herbal medicine in conjunction with certain vitamins and amino acids promote significant change in pathologic sperm. We have had excellent results in improving sperm count, improving morphology and motility as well as reducing sperm DNA fragmentation percentages.
The importance of this cannot be overstated. In the typical IVF Center, doctors will note that sperm quality is important in that in its pathological state in may not be able to penetrate the egg and facilitate fertilization. This can be overcome with intra-cytoplasmic-sperm-injection or ICSI. While fertilization with ICSI is possible, a good embryo quite often is not produced and pregnancy does not ensue.
A ‘good’ embryo is created by combining a ‘good’ egg with ‘good’ sperm. Embryos created with poor quality sperm even if fertilization occurs via ICSI are, in my opinion, based on thirteen years of clinical experience, one of the main reasons for failed IVF cycles including donor egg transfers even though fertilization with ICSI is facilitated.
Frequently the doctor will report that the embryo is “beautiful”, yet the cycle fails. You can no more judge the quality of an embryo by visualization than one can judge the health of a person by visualization. For example, the healthiest looking man can be HIV positive. Or, the healthiest looking woman can have breast cancer. One cannot judge a book by its cover.
The best and most reliable means of improving fertility outcomes either as a result of intercourse, IUI, or IVF is to first improve the quality of the components that are responsible for the creation of the embryo; sperm and egg.
See studies below for more information.
Fertility and Sterility_ Vol. 92, No. 4, October 2009 0015-0282/09/$36.00
Copyright ยช2009 American Society for Reproductive Medicine, Published by Elsevier Inc. doi:10.1016/j.fertnstert.2009.02.041
Jian Pei, Ph.D.,a,b Erwin Strehler, M.D.,b Ulrich Noss, M.D.,c Markus Abt, Ph.D.,d
Paola Piomboni, Ph.D.,e Baccio Baccetti, Ph.D.,e and Karl Sterzik, M.D.b
a Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; b Christian-
Lauritzen-Institut, Ulm, Germany; c In Vitro Fertilization Center Munich, Munich, Germany; d Institute for Mathematics,
University of Augsburg, Augsburg, Germany; and e Department of Paediatrics, Obstetrics and Reproductive Medicine, Section
of Biology, Siena University, Siena, Italy
Saturday, October 24
Poor Sperm Quality Equals Poor Embryo Quality
At the Berkley Center for Reproductive Wellness we have been treating male factor infertility for many years based on the knowledge of 3000 years of traditional Chinese medicine. Acupuncture and herbal medicine in conjunction with certain vitamins and amino acids promote significant change in pathologic sperm. We have had excellent results in improving sperm count, improving morphology and motility as well as reducing sperm DNA fragmentation percentages.
The importance of this cannot be overstated. In the typical IVF Center, doctors will note that sperm quality is important in that in its pathological state in may not be able to penetrate the egg and facilitate fertilization. This can be overcome with intra-cytoplasmic sperm injection or ICSI. While fertilization with ICSI is possible and often occurs, a good embryo quite often is not produced and pregnancy does not ensue.
A ‘good’ embryo is created by combining a ‘good’ egg and a ‘good’ sperm. Embryos created with poor quality sperm are, in my opinion, based on thirteen years of clinical experience, one of the main reasons for failed IVF cycles including those undergoing donor egg transfers even though fertilization with ICSI is facilitated.
Frequently the doctor will report that the embryo is “beautiful”, yet the cycle fails. You can no more judge the quality of an embryo by visualization than one can judge the health of a person by visualization. For example, the healthiest looking man can be HIV positive. Or, the healthiest looking woman can have breast cancer. One cannot judge a book by its cover.
The best and most reliable means of improving fertility outcomes either as a result of intercourse, IUI or IVF is to first improve the quality of the components that are responsible for the creation of the embryo; sperm and egg.
The importance of this cannot be overstated. In the typical IVF Center, doctors will note that sperm quality is important in that in its pathological state in may not be able to penetrate the egg and facilitate fertilization. This can be overcome with intra-cytoplasmic sperm injection or ICSI. While fertilization with ICSI is possible and often occurs, a good embryo quite often is not produced and pregnancy does not ensue.
A ‘good’ embryo is created by combining a ‘good’ egg and a ‘good’ sperm. Embryos created with poor quality sperm are, in my opinion, based on thirteen years of clinical experience, one of the main reasons for failed IVF cycles including those undergoing donor egg transfers even though fertilization with ICSI is facilitated.
Frequently the doctor will report that the embryo is “beautiful”, yet the cycle fails. You can no more judge the quality of an embryo by visualization than one can judge the health of a person by visualization. For example, the healthiest looking man can be HIV positive. Or, the healthiest looking woman can have breast cancer. One cannot judge a book by its cover.
The best and most reliable means of improving fertility outcomes either as a result of intercourse, IUI or IVF is to first improve the quality of the components that are responsible for the creation of the embryo; sperm and egg.
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